Non-Negative Matrix Analysis for Effective Feature Extraction in X-Ray Spectromicroscopy

TitleNon-Negative Matrix Analysis for Effective Feature Extraction in X-Ray Spectromicroscopy
Publication TypeJournal Article
Year of Publication2014
AuthorsMak, R, Lerotic, M, Fleckenstein, H, Vogt, S, Wild, SM, Leyffer, S, Sheynkin, Y, Jacobsen, C
JournalFaraday Discussions
Volume171
Date Published04/2014
Other NumbersANL/MCS-P5099-0214
AbstractX-ray absorption spectromicroscopy provides rich information on the chemical organization of materials down to the nanoscale. However, interpretation of this information in studies of “natural” materials such as biological or environmental science specimens can be complicated by the rich mixtures of spectroscopically complicated materials present. We describe here the shortcomings that some times arise in previously employed approaches such as cluster analysis, and we present a new approach based on non-negative matrix approximation (NNMA) analysis with both sparseness and cluster-similarity regularizations. In a preliminary study of the large-scale biochemical organization of human spermatozoa, NNMA analysis delivers results that nicely show the major features of spermatozoa with no physically erroneous negative weightings or thicknesses in the calculated image.  
DOI10.1039/C4FD00023D
PDFhttp://www.mcs.anl.gov/papers/P5099-0214.pdf